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. 2021 Apr 21;22:288. doi: 10.1186/s12864-021-07595-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Developmental dynamics revealed by the rumen transcriptome. a From the host rumen transcriptome, two stages were identified that transition earlier than the microbial gene abundance of metagenomics by PC1 of PCA analysis and unsupervised hierarchical clustering analysis. The black arrow represented the granulated feed has been added at d 25. b ANOSIM test of rumen transcriptomes between the two phases. If the median line of the “combined” group (including data for samples collected during both Phase 1 and Phase 2) was higher than the median lines for the two separate phases, the grouping was considered reasonable, and if the R value was greater than 0, there was a significant difference between the groups. In contrast, an R value less than 0 indicated that the difference within groups was greater than that between groups. c Barplots showing the numbers of upregulated DEGs (colored in orange) and downregulated DEGs (colored in blue) between every two time-points. d GO analysis of rumen DEGs during the first phase (colored in orange) and the second phase (colored in blue). Only terms with Bonferroni-adjusted P < 0.05 were considered