Skip to main content
. 2021 Apr 21;190:105078. doi: 10.1016/j.antiviral.2021.105078

Fig. 2.

Fig. 2

Improvement of the CoV-RdRp-Gluc reporter assay. (A) Effect of different ratios of nsp12, nsp7 and nsp8 on CoV-Gluc expression.HEK 293T cells were transfected with nsp12, nsp7 and nsp8 plasmid DNA. Levels of Gluc in the culture supernatant were measured, and the level from transfection of CoV-Gluc alone was arbitrarily set at 1. Data shown are the average of three independent experiments. (B) Expression of CoV-Gluc at different time intervals after transfection of HEK293T cells with nsp12, nsp7 and nsp8 at the ratio of 1:3:3. (C) Determination of the Z′ factor of the CoV-RdRp-Gluc reporter assay.