Fig. 3.

Dose-dependent inhibition CoV-Gluc. HEK293T cells were transfected with CoV-Gluc, nsp12, nsp7, and nsp8 plasmid DNA at the ratio of 1:10:30:30. 12 h post transfection, cells were re-seeded in 96-well plates (10⁴/well), and treated with serially diluted Remdesivir (A), Ribavirin (B), Favipiravir (C), Penciclovir (D), Entecavir (E), Tenofovir (F). After 24 h incubation, Gluc activity in supernatants was determined. To assess cell viability, HEK293T cells (10⁴/wells) were seeded in 96-well plates, and treated with these inhibitors as indicated above. The CC₅₀ values were measured with the cell counting kit-8. Results shown are the average of three independent experiments.