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Inhibition of CoV-Gluc RNA expression by NAs. HEK293T cells were transfected with CoV-Gluc, nsp12, nsp7, nsp8 plasmid DNA at the ration of 1:10:30:30. 6 h post transfection, supernatants were replaced with fresh medium containing Remdesivir (A), Ribavirin(B), Favipiravir(C), Penciclovir(D), Entecavir(E), Tenofovir(F), respectively. Cells were cultured for 24 more hours, total cellular RNA was extracted, levels of CoV-Gluc were determined by real-time RT-PCR.
