Fig. 5.

Effect of nsp14 and nsp10 on the responses of CoV-RdRp-Gluc to NA inhibitors. (A, B) HEK293T cells were transfected with CoV-Gluc, nsp12, nsp7, nsp8, nsp10, and nsp14 plasmid DNA at the ratio of 1:10:30:30:10:90. At 48 h post transfection, cell lysates were analyzed by Western blots using anti-Flag or anti-β-actin antibodies (A). Gluc activity in supernatants was determined and the results are shown in (B). (C) HEK293T cells were transfected with plasmids as described above. At 12 h post transfection, cells were re-seeded in 96-well plates (10⁴/well), and treated with serially diluted NA inhibitors including Remdesivir, Ribavirin, Favipiravir, Penciclovir, Entecavir, and Tenofovir. After 24 h, Gluc activity in supernatants were quantified. Results shown are the average of three independent experiments.