Fig. 6.

The nsp14 mutant does not affect NA inhibition of CoV-RdRp-Gluc reporter. (A, B) HEK 293T cells were transfected with CoV-Gluc, nsp12, nsp7, nsp8, nsp10, nsp14 or nps14 (D90A&E92A) at the ratio of 1:10:30:30:10:90. Western blotting was performed with anti-Flag or anti-β-actin antibodies, results are shown in (A). Gluc activity in supernatants was measured, results are shown in (B). (C) At 12 h post transfection, cells were re-seeded in 96-well plates (10⁴/wells), and treated with NA inhibitors Remdesivir, Ribavirin, Favipiravir, Penciclovir, Entecavir, or Tenofovir. After 24 h, supernatants were analyzed for Gluc activity. Results shown are the average of three independent experiments.