Table 4.

Structures and EC₅₀ Values for MMV676477 Structural Analogs^a

Compound ID	Structure	Amastigote EC50 ± SE (nM)	Promastigote EC50 ± SE (nM)	RAW 264.7 cells CC50 ± SE (nM)
	R =
MMV676477	2-SCH3	79 ± 8.4	2400 ± 35	2200 ± 410
SW100	H	1100 ± 84	2000 ± 130	690 ± 140
SW22		160 ± 3.2	6700 ± 53	1300 ± 42
SW72	4-OCH3	180 ± 8.2	7500 ± 880	> 10,000
SW41	3-CI, 4-F	20 ± 0.9	780 ± 29	2400 ±100
SW73	2-CF3	68 ± 3.9	3200 ± 87	1500 ± 140
	R =
SW75	H	290 ± 22	11200 ± 260	9900 ± 2800
SW23	−C(O)CH3	> 3000	> 50,000	> 10,000
SW101	−C(O)NH-(4-CH3-C6H4)	1100 ± 91	8200 ± 1600	3000 ± 250
SW102	−C(O)NH-(4-OCH3-C6H4)	2400 ± 160	13000 ± 1400	5200 ± 380
SW74	−C(O)-(3-pyridyl)	250 ± 40	9200 ± 1300	4200 ± 520
SW10		> 5000	> 50,000	> 10,000

^a${\text{EC}}_{50}^{72\mathrm{h}}$ and ${\text{CC}}_{50}^{72\mathrm{h}}$ values for structural analogs of MMV676477 against L. amazonensis axenic amastigotes and promastigotes and the Mϕ cell line RAW 264.7 are shown. Shown are mean ${\text{EC}}_{50}^{72\mathrm{h}}$ or ${\text{CC}}_{50}^{72\mathrm{h}}$ values (nM) calculated from three biological replicates ± SE. Structure of each compound is provided. See Supplemental Methods for synthesis of these analogs.