Fig. 1.

Upon TLRs stimulation, human CF show only TLR3 expression, while the IHCM does not express TLR2, TLR3, and TLR4. (a-c) Human CF and IHCM were stimulated for 2, 4, 8, and 24 h with 10 ng/ml Pam3CSK4 (a), with 10 ng/ml PolyIC (b), or with 20 ng/ml LPS (c). Transcription of TLR2, TLR3, and TLR4 was determined by real-time RT-PCR. Dashed line set at “1” represents unstimulated cells. Means ± s.d. and values measured from one out of three independent experiments performed in duplicates are shown