Fig. 6.

Human CF, and partially the IHCM, induce IFN-β expression after intracellular PolyIC stimulation. (a) Human CF and IHCM were stimulated for 2, 4, 8, and 24 h with 1 ng/ml of intracellularly transfected PolyIC. Transcription of IFN-β, determined by real-time RT-PCR, was increased in both human CF and in the IHCM. (b) Knockdown of TLR3, MDA5, and RIG-I with siRNA in human CF stimulated for 2, 4, and 8 h with 1 ng/ml of intracellularly transfected PolyIC. SiMock was used as a control. Transcription of IFN-β, which was determined by real-time RT-PCR, was MDA5-, RIG-I-, und TLR3-dependent. Means ± s.d. and values measured from one out of three independent experiments performed in duplicates are shown. Two-way ANOVA with Bonferroni post hoc testing, ***p < 0.001 for siMock vs. siTLR3, siMock vs. siMDA5, and siMock vs. siRIG-I (b)