Figure 5.

Heritable prion-like aggregation of candidate proteins. (a) Proteins of interest were expressed as C-terminal eGFP fusions under an inducible galactose promoter in a low copy number (CEN) plasmid in the laboratory strain BY4741. Strains were grown overnight to stationary phase in SRaffinose-URA. Images from the overnight culture were taken as a ‘pre-induction’ view. The strains were then washed and diluted to OD₆₀₀ = 0.1 in 2% galactose (inducer). After 10 h, ‘induced’ images were taken. The strains were then washed and diluted by 200-fold in 2% raffinose and imaged after 24 h of ‘outgrowth’, by which time they had achieved an OD₆₀₀ of approximately 1.0. (b) Quantifications of the mean fraction of cells with puncta in each strain. Each point represents a result from an independent experiment where we counted on average approximately 150 cells per strain. Error bars represent standard error determined from three independent experiments. (Online version in colour.)