Table 2.

Top 20 most abundant protein components of S. japonicum egg ESPs. The method of protein secretion as predicted by SecretomeP is listed; classically secreted (Y), non-classically secreted (A) or non-secreted (N). Proteins are ranked in order of the highest total unique matched peptides taken from three technical replicates.

Accession Number	Protein ID	# Unique Peptides	% Coverage	SecretomeP
Q5DET3	Histone H4	19	71.8	N
Q5DFZ8	Fructose-bisphosphate aldolase	16	55.8	N
Q86G46	Polyubiquitin	16	32.1	N
C1L4Z7	Actin 5C	13	33.2	N
Q5DHF8	Histone H3	12	64.5	A
C1LFC4	Aminopeptidase	10	18.2	A
C1L9B2	Calreticulin	8	19.8	Y
Q5DDU9	Histone H2B	7	38.0	N
C1LEJ4	Histone H2A	7	46.4	A
Q86EU4	Cytochrome c proximal	7	50.8	A
C1LQJ9	Uncharacterized protein	6	23.3	A
C1LQT3	Thioredoxin	6	53.5	A
Q5D8W0	SJCHGC06710 protein	6	40.8	Y
P33676	Enolase	6	16.0	A
Q5D9T1	Malate dehydrogenase	6	27.1	A
C1LJG7	NADH:ubiquinone oxidoreductase	5	22.0	A
C1LYI9	Calcium-binding EF-hand protein	5	58.7	A
C1LNR0	Protein disulfide-isomerase	5	14.5	Y
C1LV44	Tryparedoxin peroxidase	5	28.1	A
C1LNY3	Superoxide dismutase [Cu-Zn]	5	62.6	A