Video 2. Side-by-side depiction of fluorescence, fluorescence lifetime imaging, and cell-based phasor data of intravitally imaged germinal center (day 8 p.i.), single z-plane.

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Left: fluorescence data with antigen (AG)-specific YellowCaB cells (green) and stained non-AG-specific YellowCaB cells transferred 1 day prior to imaging (red; autofluorescence of capsule also visible in the same channel). 4 fps, scale bar 50 µm. Middle: false color-coded presentation of fluorescence lifetime τ (0–3000 ps, see range scale in Figure 5b). 4 fps, scale bar 50 µm. Right: raw cell-based phasor plot with cells segmented according to fluorescence and spatial distribution, subsets indicated. 4 fps.