Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Mouse | R26CAG-TNXXLflox | Mank et al., 2008 | - | Plasmid available at addgene (#45797) |
| Mouse | CD19 cre | Rickert et al., 1997 | Stock# 004126 jaxmice |
|
| Mouse | B1-8high | Shih et al., 2002a | Stock# 007775 jaxmice |
|
| Antibody | Rabbit anti-GFP-Alexa488 polyclonal |
Rockland, in-house coupling DRFZ Berlin |
Cat# 600-401-215 | 1:200 |
| Antibody | Rat anti-CD21/35-Fab-Atto590 7G6 |
DRFZ Berlin | – | 10 µg/mouse |
| Antibody | Hamster anti-IgD-Alexa594 11.26 c |
DRFZ Berlin | –- | – |
| Antibody | Rat anti-CD21/35-Alexa647 7G6 |
DRFZ Berlin | – | – |
| Antibody | Rat anti-CD19-Cy5 1D3 |
DRFZ Berlin | – | – |
| Antibody | Rat anti-CD40 3/23 |
BD Pharmingen | Cat# 553788 | As indicated |
| Antibody | Goat anti-IgM-F(ab)2 polyclonal |
Southern Biotech | Cat#1023-01 | As indicated |
| Antibody | Rat anti-kappa 187.1 |
DRFZ Berlin | – | As indicated |
| Recombinant DNA reagent | CpG | Tib Molbiol Berlin |
Request #1668 and 1826 sequence ID: 1746437/8 |
As indicated |
| Peptide, recombinant protein | Peanut-agglutinin, biotinylated | Vector Biolabs | Cat# B-1075-5 | 1:200 |
| Other | Lipopolysaccharide (LPS) from Escherichia coli | Sigma | Cat# L4391 | As indicated |
| Peptide, recombinant protein | NP-CGG ratio > 20 | Biosearch Technologies | Cat# N-5055C-5-BS |
10 µg/mouse |
| Peptide, recombinant protein | Streptavidin-Alexa555 | Thermo Fisher Scientific | Cat# S32355 | 1:2000 |
| Other | X-Rhod-1 | Molecular Probes | Cat# X14210 | Manufacturer’s protocol |
| Software, algorithm | Python 2.7 | Python Software Foundation | ||
| Software, algorithm | MATLAB | MathWorks | ||
| Software, algorithm | Imaris | Bitplane | ||
| Software, algorithm | Phasor-based-numerical analysis of eCFP lifetime data | Own work, https://github.com/ulbrica/Phasor-FLIM.git; swh:1:rev:8ae5bfc17ec019fcc8ec7e4627442646e52cc3c5; Ulbricht, 2021. |