Table 2.

The time program of gradient elution.

Time (min)	A (%)	B (%)
0–12	90–70	10–30
12–17	70	30
17–20	70–42	30–58
20–23	42–40	58–60
23–27	40–30	60–70
27–32	30–15	70–85
32–35	15–5	85–95
35–45	5	95