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. 2021 Apr 14;2021:6660486. doi: 10.1155/2021/6660486

Figure 1.

Figure 1

Id4 overexpression reduces cell proliferation and arrests cell cycle at the G0/G1 phase. (a) Id4 protein expression in the CRC. (b, c) Real-time RT-PCR and Western blotting confirmed stable Id4 overexpression in the HCT116 cells. β-Actin was used as a loading control. (d) WST assay was used to assess the effect of Id4 on the proliferation of HCT116 cells. (e) Colony formation assay was used to assess the effect of Id4 on the colony-forming ability of the HCT116 cells. (f) Flow cytometry was performed to detect the cell cycle after Id4 overexpression. (g) Photographs of the xenograft tumors in the nude mice at day 14 after implantation, and tumor weight was measured. (h) Protein expression of molecules in the PI3K/AKT pathway and proliferation-related proteins in the HCT116-Id4 cells (Western blotting). GAPDH was used as a loading control. Data are expressed as mean ± SD. ∗P < 0.05.