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. 2021 Apr 21;6:59. doi: 10.1038/s41541-021-00320-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Thermostable LAIV was stored at 25 °C and 37 °C, and liquid LAIV was stored at −80 °C for up to 52 weeks. T-LAIV was reconstituted with 1 ml PBS and infectious titer was determined by titration in embryonated chicken eggs. T-LAIV stability at each time point was compared to liquid LAIV stored at −80 °C (black circles). Two-way ANOVA: *p < 0.05, **p < 0.01, ****p = 0.0001; ns, not significant. Error bars indicate mean ± 95% CI. n = 3.