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. 2021 Apr 21;11:8576. doi: 10.1038/s41598-021-87886-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fungal root colonization and plant growth of mycorrhizal (Myc) and non-inoculated (Cont) cork oak plants, 8 weeks after P. tinctorius inoculation. (a) Ergosterol root content. Data represent the mean of 3 independent biological replicates (each composed of a pool of 3 individual root systems) ± SD (b) Root fresh weight. Data represent the mean of 15 independent plants ± SD. *Indicates statistical differences at the level P < 0.05 [t-test for (a) and Man-Whitney U test for (b)]. (c) Colonized root showing the hyphal mantle. Scale bar = 1 mm. (d) Representative trypan blue staining of a colonized root showing the fungal mantle (m) and the hartig net (hn). Scale bar = 50 μm.