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. 2021 Apr 21;11:8585. doi: 10.1038/s41598-021-87949-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Netrin-1 inhibits BMP signaling in various mammalian cell lines. Western blot analysis showing the inhibitory effect of netrin-1 on the BMP4-induced pSmad1/5 levels in cell lines of different origins. (a) MEFs and human embryonic kidney (HEK293) cells. (b) Human ovarian cancer (Ovcar3 and Ovsaho), malignant melanoma (A375), breast cancer (MDA-MB-415 and T-47D), glioblastoma (TB101 and TB107), colorectal carcinoma (HCT116 and LoVo), and acute lymphoblastic pre-B cell leukemia (697 and RCH-ACV). Representative Western blots of pSMAD1/5 and actin after the cells were treated with BMP4 and netrin-1 are shown together with the quantifications of the pSMAD1/5/actin ratios. Uncropped blots are shown in Fig. S4. Shown are the means and individual values of four independent experiments (Student’s t-test: ^NSp ≥ 0.05; *p < 0.05; **p < 0.01).