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. 2021 Apr 21;12:2357. doi: 10.1038/s41467-021-22444-1

Fig. 1. Analytical characterization of cell-free RNA.

Fig. 1

a Fragment Analyzer (Agilent) trace of cfRNA fragment lengths in a non-cancer sample following deoxyribonuclease (DNase) digestion. Inset: Relative proportion of different RNA types found by whole-transcriptome sequencing in a representative non-cancer sample prior to abundant transcript depletion. Sequencing coverage across a 36 kb region of b PIK3CA, a high-abundance cell-free RNA gene, and c EGFR, a low-abundance cell-free RNA gene, from a representative patient sample. Blue bars represent coverage for a whole-transcriptome cfRNA sample with 584 M paired-end reads, and red bars represent coverage for a whole-genome cfDNA sample from the same patient with 871 M paired-end reads. The dashed line represents median cfDNA coverage.