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. 2021 Apr 21;11:8672. doi: 10.1038/s41598-021-87986-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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EV collection using the AGC device. (a) A schematic illustration of EV collection using the AGC device and the evaluation of the collection efficiency using a plate reader and fluorescence microscope. (b) A TEM image of EVs derived from HepG2 cells (HepG2-EVs). (c) The size and zeta potential distribution of HepG2-EVs. (d) A fluorescence image of an MPG membrane after the collection of HepG2-EVs labeled with PKH67 (green fluorescence) using the AGC device. (e,f) The capture rate (%) of HepG2-EVs by MPG membranes of different pore size (e) and thickness (f). (g) A SEM image of HepG2-EVs collected in MPG membranes with different pore sizes. (h) The schematic illustration demonstrating the depth at which HepG2-EVs could be observed in the MPG membrane with different pore sizes.