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. 2021 Apr 21;11:8672. doi: 10.1038/s41598-021-87986-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of four EV collection methods. (a) A schematic diagram of the real-time PCR analysis of has-miR-21 extracted from HepG2-EVs collected from cell culture supernatant using four EV collection methods (ultracentrifugation [UC], ExoCap [MB], ExoQuick [PR] and the AGC device). (b) The isolation time of HepG2-EVs in UC, MB, PR and the AGC device. (c) The detected level of hsa-miR-21 in UC, PR, MB and the AGC device. (d) The detected level of hsa-miR-21 in the AGC device with different volumes of cell culture supernatant.