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. 2021 Apr 21;11:8672. doi: 10.1038/s41598-021-87986-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The miRNA array analysis of EVs collected from human urine. (a) A gel image of nucleotides [nt] of total RNA extracted from EVs collected from human urine using four EV collection methods (UC, MB, PR, and the AGC device) measured by a 2100 Bioanalyzer. (b) The concentration of total RNA extracted from EVs collected from human urine using four EV collection methods (UC, MB, PR, and the AGC device) measured by a 2100 Bioanalyzer. (c) The number of miRNAs extracted from EVs collected from human serum using four EV collection methods (UC, MB, PR, and the AGC device). (d) A venn diagram of the overlap of the number of detected miRNAs between UC (as the standard method) and the other three methods (MB [d], PR [e] and the AGC device [f]).