Skip to main content
. 2021 Apr 21;4:490. doi: 10.1038/s42003-021-02003-6

Fig. 7. Partial EMT induced by αvβ3 integrin is independent of TGF-β signaling.

Fig. 7

a Western blotting of phospho-Smad2, phospho-Smad3, and Smad2/3 in control and β3-A549 cells pretreated with or without 5 µM SB431542 for 20 min and then treated with 2 ng/ml TGF-β1 for 30 min either separately or in combinations. b Anti-Smad2/3 immunofluorescence staining (green) of β3-A549 cells treated with DMSO, 2 µM SB431542, and 4 ng/ml TGF-β1 for 2 days either separately or in combinations. Nuclear staining (blue) was used to detect nuclei and the co-localization of nuclei with Smad2/3 in the merged panel. Scale bar, 25 µm. c Cell morphology of control and β3-A549 cells pretreated with or without 5 µM SB431542 for 20 min at room temperature and then treated with 5 ng/ml TGF-β1 for 17 h either separately or in combinations. Scale bar, 40 µm. d Western blotting of EMT markers in control and β3-A549 cells pretreated with or without 5 µM SB431542 for 20 min at room temperature and then treated with 5 ng/ml TGF-β1 for 2 days either separately or in combinations. Unprocessed blot images in (a and d) are shown in Supplementary Fig. 9.