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. 2021 Apr 21;4:490. doi: 10.1038/s42003-021-02003-6

Fig. 8. miR-200 family can prevent αvβ3 integrin-mediated partial EMT.

Fig. 8

a Cell morphology of control A549 cells, β3-A549 cells, and control and β3-A549 cells stably expressing the miR-200 family (β3-miR200-A549 cells) with or without 2 ng/ml TGF-β1 treatment for 24 h. Scale bar, 100 µm. b Relative mRNA expression levels (qRT-PCR) of EMT markers in β3-A549 and β3-miR200-A549 cells. One-way ANOVA and Bonferroni post-hoc test, mean ± s.e.m. of three independent assays conducted in triplicate. *P = 4.2 × 10−11, **P = 7.6 × 10−10, ***P = 2.1 × 10−8. c Western blotting of EMT markers in β3-A549 and β3-miR200-A549 cells with or without 2 ng/ml TGF-β1 treatment for 24 h. d Effect of the miR-200 family expression on the cell migration of β3-A549 cells. Two-tailed unpaired Student’s t-test, mean ± s.e.m. of three independent assays conducted in triplicate. e Western blotting analysis of the intracellular expression levels of αv and β3 integrins in control, β3- and β3-miR200-A549 cells. f FACS analysis of the cell surface expression of αvβ3 integrin in control, β3-, and β3-miR200-A549 cells. The source data are provided in Supplementary Data 1. Unprocessed blot images in (c and e) are shown in Supplementary Fig. 10.