Figure 1.

The activity state of BSRC affects peripheral CD8⁺ T_EMRA cells. (A) Exemplary strategy of gating CD8⁺ T-cell stages: T_CM (CCR7⁺CD45RA⁻), T_EM (CCR7⁻CD45RA⁻), naïve (CCR7⁺CD45RA⁺), and T_EMRA (CCR7⁻CD45RA⁺). (B) Blood analysis of peripheral CD8⁺ T_EMRA cells from controls compared to disease-active and -inactive BSRC patients. Lines indicate the median. Statistical analysis was performed using Student’s t-test with Welch’s correction (p < 0.05*). (C) Volcanoplot (− log10) shows Spearman’s Rho correlation coefficient of immunological versus clinical parameters of significant altered CD4⁺ and CD8⁺ T-cell populations in BSRC patients. (D) Linear regression of CD8⁺ T_EMRA cells with age of active and inactive BSRC patients and control probands. The population of CCD7−/CD45RA− was in one inactive patient absent, therefore the (B) and (D) include n = 5 inactive patients. BSRC birdshot retinochoroiditis, CM central memory, CME cystoid macula edema, CRT central retinal thickness, EM effector memory, Freq. frequency, NS not significant, TEMRA terminally differentiated effector memory CD45RA⁺ T cells.