Table 1.

Purification profile of SSTI.

Steps	Total protein (mg)	Total activity (TIU)	Specific activity (TIU/mg)	Yield %	Fold purification
Crude extract	860	9971	11.5	100	1
After dialysis	475.2	6078	13	55	1.2
Ion exchange chromatography	4.83	498	103.1	0.56	9

Total amount of protein extracted from 10 g of fine powder of dried seeds of S. surattense.

TIU (trypsin inhibitory activity unit) was defined as the decrease in 0.01 unit of absorbance at 410 nm.