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. Author manuscript; available in PMC: 2021 Oct 13.
Published in final edited form as: Mol Psychiatry. 2020 Oct 22;26(7):3178–3191. doi: 10.1038/s41380-020-00905-1

Fig. 5. MSDB-specific CB1 or MAGL knockout has opposing effects on anxiety- and depressive-like behavior.

Fig. 5.

a. Timeline of AAV injection and behavior tests. b. Open field distance traveled was not significantly altered by MSDB knockout (F2,34 = 1.524, p = 0.232). Center time in the open field was significantly decreased in MSDB-CB1-KO mice relative to MSDB-WT and MSDB-MAGL-KO mice (F2,34 = 7.169, p = 0.003; MSDB-CB1-KO vs. MSDB-WT, p = 0.028; MSDB-CB1-KO vs. MSDB-MAGL-KO, p = 0.002). The number of center entries was significantly reduced in MSDB-CB1-KO mice relative to MSDB-WT and MSDB-MAGL-KO mice (F2,34 = 8.207, p = 0.001; MSDB-CB1-KO vs. MSDB-WT, p = 0.004; MSDB-CB1-KO vs. MSDB-MAGL-KO, p = 0.003). The percent distance traveled in the center was significantly reduced in MSDB-CB1-KO mice relative to MSDB-MAGL-KO mice, but was not significantly altered in MSDB-CB1-KO mice relative to MSDB-WT mice (F2,34 = 4.571, p = 0.017; MSDB-CB1-KO vs. MSDB-WT, p = 0.056; MSDB-CB1-KO vs. MSDB-MAGL-KO, p = 0.020). MSDB-WT: n = 12; MSDB-CB1-KO: n = 11; MSDB-MAGL-KO: n = 14. c. Marble burying behavior was increased in MSDB-CB1-KO mice (F2,34 = 8.076, p = 0.001; MSDB-CB1-KO vs. MSDB-WT mice, p = 0.015; MSDB-CB1-KO vs. MSDB-MAGL-KO mice, p = 0.002). d. Novelty-suppressed feeding changes induced by MSDB-specific CB1 or MAGL KO (H = 22.461, p < 0.001; MSDB-CB1-KO vs. MSDB-WT, p = 0.0035; MSDB-CB1-KO vs. MSDB-MAGL-KO, p < 0.001). Home cage feeding behavior was not significantly altered between groups (H = 6.149, p = 0.046; MSDB-WT vs. MSDB-CB1-KO, p = 0.0803; MSDB-WT vs. MSDB-MAGL-KO, p > 0.9999; MSDB-CB1-KO vs. MSDB-MAGL-KO, p = 0.1295). e. Sucrose preference increased in MSDB-MAGL-KO mice (F2,34 = 6.060, p = 0.006; MSDB-MAGL-KO vs. MSDB-WT mice, p = 0.026; MSDB-MAGL-KO vs. MSDB-CB1-KO mice, p = 0.011). (c-e) MSDB-WT: n = 9; MSDB-CB1-KO: n = 13; MSDB-MAGL-KO: n = 15.