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. 2021 Apr 21;11:8626. doi: 10.1038/s41598-021-88133-7

Figure 1.

Figure 1

Tiliroside inhibited the proliferation and colony formation of Hep3B and SNU-449 cells and the restoration by CAXII overexpression. Relative inhibition rates of Hep3B, SNU-449 and also THLE-3 in response to different concentrations of Tiliroside were calculated by comparing the OD value of NC, at 24, 48, 72, and 96 h, respectively (A–C). The representative colony formation of Hep3B and SNU-449 cells intervened by 40 μM Tiliroside and NC (H). The relative colony formation efficiency showed significant reduction of colony 10 days after Tiliroside intervention in both Hep3B and SNU-449 cells (F). The relative CAXII expression was significantly higher in CAXII-transfection group than non-transfection group in both Hep3B and SNU-449 cell lines (D). The relative CAXII expression was significantly decreased in CAXII-KO group than none transfection group in both Hep3B and SNU-449 cell lines (E). The inhibition rates brought by Tiliroside were significantly increased in CAXII overexpression group compared to both non-transfection group and empty vector group, at 48 and 72 h, respectviely, in Hep3B and SNU-449 cells. On the contrary, The inhibition rates were significantly decreased in CAXII-KO group compared to both non-transfection group and none-KO group, at 24, 48 and 72 h, respectviely in both cell lines (E). Data are presented as the mean ± standard deviation (SD); NS not significant, NC negative control.