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. 2021 Apr 21;11:8626. doi: 10.1038/s41598-021-88133-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Tiliroside elevated the activity of Caspase-3. After 48 h intervention of 40 μM Tiliroside or DMSO, the absorbance was significant higher in the Tiliroside group than NC, apoptosis inhibited NC and apoptosis inhibited Tiliroside group in Hep3B (A) and SNU-449 (B), cultured in either 2D or 3D systems, respectively. CASP3-specific activities were also significantly higher in Tiliroside treated group compared to NC in Hep3B, SNU-449 and THLE-3 cells (C); NC negative control, NS non-significant.