Table 1.

Primer sequences and amplification characteristics of candidate reference genes of S. glauca used in this study.

Accession number	Arabidopsis ortholog locus	Identity (%)	Gene Name	Primer sequence 5′–3′ (F)	Amplicon length	Efficiency (%)	R2
BE859265.1	AT5G09810	0.86	ACT7	F: TAATCATCAAAATCCTGAGGA	103	119	0.9972
				R: ATTATGGTATGTAATCTTTGCGG
MF893334.1	AT3G12110	0.81	ACT11	F: TGTTGCTCCAGAAGAGCATC	116	96	0.9969
				R: CATACATGGCAGGGACATTG
BF114443.1	AT3G63520	0.85	CCD1	F: CCCAATCAAGGGTTCACTTC	108	107	0.9988
				R: GTGTTGAGGTTGTGAAGAATCA
AW990992.1	AT5G19780	0.86	TUA5	F: GGCACAATGCACTAAGCAAC	110	98	0.9991
				R: AAGGTGCCGAGGATGATGAT
BE240972.1	AT1G55860	0.84	UPL1	F: GAGTGGTACCAGCTATTGTC	103	114	0.9994
				R: AGACAGAATTAGGGTTTGGCT
BE859200.1	AT1G64230	0.81	UBC28	F: CCCTCCAGATTATCCATTTAAG	106	–	–
				R: CTCCTTTAGGATGTCAAGACA
BE644594.1	AT5G60390	0.91	EF1α	F: AGACCAACAAGTACTACTGCA	108	–	–
				R: CAATAATAAGGATAGCGCAGTC
BE240909.1	AT1G69960	0.78	PP2A	F: CTTAGTATTCCCATTTCTTCATCT	144	104	0.9975
				R: ATGAGGACACAAAAAGAGCCAT
KM679415.1	AT5G51990	0.71	DREB1D	F: CGACAGACACTAGGGAAATTC	107	95	0.9923
				R: GTCATTCATGCTGCTATTCTC
BE231385.1	AT2G21170	0.77	TIM	F: GTTGTTACTATGGCTGGCTC	111	–	–
				R: CAATGTTGCACTGTTCAAGTCT
BF145083.1	AT3G42050	0.77	V-H + -ATPase	F: CTAGCATAATTTCTGCAAAGCC	119	107	0.994
				R: CTGTTCCAGTGATCAACTTAGT
BE656716.1	At2g43790	0.81	MPK6	F: CAACCTCATTCATCAGTCATCA	112	87	0.9763
				R: TGGTTTGCGGTGGTTGATTAG
AW982148.1	AT5G20380	0.75	PHT4;5	F: AGCAACAGCATTCGTTCCAG	106	106	0.9983
				R: GATCTGTGGCAGCTGATGGT
				R: AACTTCAAAGGCAATGTTGAAAAC
BE644575.1	At5g52660	0.85	MYB	F: CTGCTAATGTGGTGTCACCAT	114	98	0.9923
				R: CATGCTCTTCTTCAGTCCAAC
BE231371.1	–	–	AP2	F: AACTCTTTCTTCCTTAATCACTCT	124	95	0.9748
				R: CGTCGATAAAGTTCTCATTTTTAC

Amplicon length was obtained using cDNA as a template.