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. 2021 Apr 21;11:8569. doi: 10.1038/s41598-021-88151-5

Table 3.

Stability assessment of all candidate reference genes of S. glauca plants’ response to salinity stresses calculated using the BestKeeper algorithms.

Order A B C D E F
Gene SD coeff. of corr. [r] Gene SD coeff. of corr. [r] Gene SD coeff. of corr. [r] Gene SD coeff. of corr. [r] Gene SD coeff. of corr. [r] Gene SD coeff. of corr. [r]
1 MPK6 0.49 0.998 PP2A 0.53 0.998 TUA5 0.50 0.999 PP2A 0.53 0.999 TUA5 0.50 0.999 MPK6 0.58 0.999
2 PP2A 0.41 0.978 V-H+-ATPase 0.48 0.983 PP2A 0.44 0.980 MPK6 0.51 0.979 PP2A 0.50 0.979 PP2A 0.50 0.982
3 TUA5 0.45 0.971 TUA5 0.53 0.971 V-H+-ATPase 0.51 0.971 TUA5 0.48 0.978 MPK6 0.56 0.977 TUA5 0.53 0.979
4 CCD1 0.22 0.964 MPK6 0.30 0.966 MPK6 0.33 0.971 V-H+-ATPase 0.27 0.972 V-H+-ATPase 0.29 0.969 UPL1 0.23 0.970
5 V-H+-ATPase 0.38 0.961 ACT11 0.44 0.949 CCD1 0.46 0.966 UPL1 0.42 0.955 ACT11 0.46 0.967 ACT11 0.47 0.962
6 ACT11 0.52 0.881 CCD1 0.61 0.826 ACT11 0.60 0.874 ACT11 0.54 0.883 UPL1 0.54 0.839 ACT7 0.61 0.847
7 UPL1 0.30 0.76 UPL1 0.33 0.774 UPL1 0.41 0.729 CCD1 0.33 0.777 CCD1 0.31 0.756 CCD1 0.35 0.774
8 PHT4;5 0.53 0.718 PHT4;5 0.54 0.719 ACT7 0.64 0.723 PHT4;5 0.65 0.726 DREB1D 0.64 0.722 PHT4;5 0.63 0.627
9 ACT7 0.40 0.471 ACT7 0.49 0.472 PHT4;5 0.46 0.472 DREB1D 0.49 0.380 ACT7 0.49 0.278 V-H+-ATPase 0.43 0.472
10 DREB1D 0.28 0.083 DREB1D 0.30 0.187 DREB1D 0.40 0.289 ACT7 0.40 0.088 PHT4;5 0.28 0.085 DREB1D 0.36 0.193

A: Thirteen tissue samples derived from 1/10 MS liquid medium treatment.

B: Thirteen tissue samples derived from 100 mM NaCl treatment.

C: Thirteen tissue samples derived from 300 mM NaCl treatment.

D: Fifteen germinating seed samples derived from NaCl gradient treatment.

E: Eighteen seedling samples derived from NaCl gradient treatment.

F: All 72 samples used in this study.