FIGURE 2.

Schematic representation of diverse state-of-the-art tools for single cell analysis. Next to microfluidic based single cell sequencing methods, like Drop-seq, inDrop or 10X Genomic Chromium, for high throughput transcriptome analysis of single cells, fluorescence-based methods are used to investigate single cell transcriptomes. Further, Sequential barcoding methods like SPLIT-seq are applied for high throughput single cell sequencing. Less throughput, but higher precision was shown to be achieved using SMART-Seq methods with prior fluorescence activated cell sorting (FACS).