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. 2021 Apr 8;9:640169. doi: 10.3389/fcell.2021.640169

TABLE 1.

Primers used for cloning.

Primer Sequence
pME_sigpep-YFP-nostop
attB1_sigpep_F GGGGACAAGTTTGTACAAAAAAGCAGG CTGCCACCATGAAAGCTTCTGTCGGAG
Sigpep_YFP_F ATGAAAGCTTCTGTCGGAGTGTGTGTGGT TTTCGTGCTGGCTCTGCTGGGGCTTGGTT CTGCCatggtgagcaagggcgag
attB2R_YFP_nostop_R GGGGACCACTTTGTACAAGAAAGCTGGG Tccttgtacagctcgtccatg
p3E_CD59pA
ClaI_CD59_F GACTATCGATATTAAATGTTACAATTGTA AGGAC
XbaI_CD59_R GATCTAGATTAGAAAACACCCCACCAG
attB2_CD59_F GGGGACAGCTTTCTTGTACAAAGTGGTAA TTAAATGTTACAATTGTAAGGAC
attB3R_pA_R GGGGACAACTTTGTATAATAAAGTTG
pME_mScarlet-CAAX
attB1F_mScarlet-CAAX_F GGGGACAAGTTTGTACAAAAAAG
mScarlet-CAAX_R TCAGGAGAGCACACACTTGCAGCTCATG CAGCCGGGGCCACTCTCATCAGGAGGGT TCAGCTTCTTGTACAGCTCGTCCATG
attB2R_CAAX-mScarlet_R TCAGGAGAGCACACACTTGCAGCTCATGCA GCCGGGGCCACTCTCATCAGGAGGGTT CAGCTTCTTGTACAGCTCGTCCATG