Figure 1.

Propofol induces miR-145-5p expression in BC cells. (A) Relative expression of miR-145-5p in 30 BC samples and paired normal tissues as determined via RT-qPCR. (B) Relative expression of miR-145-5p in BC cell lines (J82 and T24) and SV-HUC-1 cells as determined via RT-qPCR. Relative expression of miR-145-5p in (C) J82 and (D) T24 cells at different time points after exposure to 10 µg/ml propofol. (E) J82 and (F) T24 cells were treated with increasing concentrations (0–20 µg/ml) of propofol for 24, 48 or 72 h, then cell viability was evaluated. Each assay was performed in triplicate and data are presented as the mean ± SD. *P<0.05. BC, breast cancer; miR, microRNA; RT-qPCR, reverse transcription-quantitative PCR.