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. 2021 Apr 9;23(6):439. doi: 10.3892/mmr.2021.12078

Figure 4.

Figure 4.

Propofol suppresses TOP2A expression in BC cells. (A) Relative TOP2A expression in 30 BC samples and paired normal tissues as determined via RT-qPCR. (B) Relative TOP2A expression between BC samples and paired normal tissues as determined via western blot analysis. (C) Relative TOP2A expression in different cell lines as determined via RT-qPCR. (D) Relative TOP2A expression in different cell lines as determined via western blot analysis. J82 and T24 cells were treated with 10 µg/ml propofol or an equal volume of DMSO as control, then relative TOP2A expression was evaluated via (E) RT-qPCR and (F) western blot analysis. (G) Spearman correlation analysis of miR-145-5p and TOP2A expression in patients with BC. Each assay was performed in triplicate and data are presented as the mean ± SD. *P<0.05. BC, bladder cancer; N, normal; T, tumor; miR, microRNA; TOP2A, topoisomerase II α; RT-qPCR, reverse transcription-quantitative PCR.