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. 2021 Apr 9;23(6):439. doi: 10.3892/mmr.2021.12078

Figure 5.

Figure 5.

Propofol exerts antitumor effects via the miR-145-5p/TOP2A axis. (A) J82 and T24 cells were transduced with anti-miR-NC or anti-miR-145-5p, then relative TOP2A expression was evaluated via RT-qPCR. (B) J82 and T24 cells were transfected with si-NC or si-TOP2A, then relative TOP2A expression was evaluated via RT-qPCR. (C) J82 and (D) T24 cells were transduced with anti-miR-NC, anti-miR-145-5p, si-NC or si-TOP2A, then treated with 10 µg/ml propofol or an equal volume of DMSO as control. Relative TOP2A expression was evaluated via RT-qPCR. (E) J82 and (F) T24 cells were transduced with anti-miR-NC, anti-miR-145-5p, si-NC or si-TOP2A, then treated with 10 µg/ml propofol or an equal volume of DMSO as control. Relative TOP2A expression was evaluated via western blotting. Each assay was performed in triplicate and data are presented as the mean ± SD. *P<0.05. miR, microRNA; si, small interfering RNA; NC, negative control; TOP2A, topoisomerase II α; RT-qPCR, reverse transcription-quantitative PCR.