Figure 6.

Propofol attenuates tumor growth via regulation of miR-145-5p/TOP2A in vivo. T24 cells were transduced with anti-miR-145-5p or anti-miR-NC lentiviral vectors and then injected into female BALB/C nude mice, which were treated with 10 mg/kg propofol for 4 weeks. (A) Tumor growth curves, (B) images of tumors and (C) tumor weights in each group. (D) Relative tumor miR-145-5p expression in different groups as determined via RT-qPCR. Relative tumor TOP2A expression in different groups as determined via (E) RT-qPCR or (F) western blotting. (G) Expression of vimentin and E-cadherin in tumor xenografts from each group was detected via immunohistochemistry; (H) relative numbers of positively stained cells were shown. Scale bar, 500 µm. Each assay was performed in triplicate and data are presented as the mean ± SD. *P<0.05. miR, microRNA; NC, negative control; TOP2A, topoisomerase II α; RT-qPCR, reverse transcription-quantitative PCR.