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Silencing of DLEU1 induces G1 arrest in Cal-27 cells. (A) The cell cycle distribution was determined by flow cytometry. (B and C) The protein expression of CDK6 in Cal-27 cells was detected via western blotting. The relative expression was semi-quantified via normalization to β-actin. *P<0.05, **P<0.01 vs. control; ##P<0.01 vs. DLEU1 siRNA2. DLEU1, deleted in lymphocytic leukemia 1; NC, negative control; siRNA, small interfering RNA; miR, microRNA.
