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miR-654-5p suppresses autophagy in intervertebral disc degeneration. (A) Monodansylcadaverine staining was performed to observe autophagic vacuoles in transfected nucleus pulposus cells. Scale bar, 20 µm. (B) Protein expression levels of LC3-II/LC3-I, ATG12, ATG13 and Beclin-1 were assessed by western blotting. *P<0.05. ATG, autophagy-related gene; miR-654-5p, microRNA-654-5p; NC, negative control.
