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Surface and intracellular (intra) immunofluorescence analysis of TGEV-S mutants (the amino acid exchange and position is indicated). BSR-T7/5 cells were transfected with the pTM1 expression plasmid containing the gene indicated. Cells were analyzed for surface and intracellular expression of the indicated proteins at 24 h post-transfection by immunofluorescence microscopy.
