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. 2021 Jan 4;279(42):43661–43666. doi: 10.1074/jbc.M407233200

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© 2004 © 2004 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.

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Fig. 4 — TGEV-S parental, mutant, and chimeric proteins were expressed and analyzed by surface biotinylation (A) or Western blot (B). BSR-T7/5 cells were transfected with the pTM1 expression plasmid containing either of the following TGEV-S genes, a, SSS; b, SFF; c, SFS; d, SSF; e, Y1440A; f, E1441A; g, P1442A; h, I1443A; i, E1444A; j, mock-transfected cells. At 24 h post-transfection cells were prepared for surface biotinylation or Western blot.