(A) STAT5a and STAT3 phosphorylation by P14 Tn, (gray), Tep (black) or Tlp (red) cells isolated from mice 60hrs after priming and then cultured without stimulation (media) or following IL-2 or IL-21 stimulation for 30 minutes.
(B & C) Naïve mice immediately infected with Cl13 or mice infected 21 days prior with Cl13 received P14 T cells and were treated with (B) isotype (gray) or anti-IL-2 (blue); (C) isotype (gray) or anti-IL-21R (red) antibodies. Flow plots depict the proportion and bar graphs the number of total, GzmB+ and TCF1+ virus-specific CD8+ Tep and Tlp P14 cells 8 days after priming.
(D) Mice were CD4+ depleted or isotype treated (undepleted) prior to Cl13 infection. (Top) Flow plots depict the proportion and bar graphs show the numbers of total, GzmB+ and TCF1+ subsets of Tep or Tlp cells 8 days after priming. (Bottom) Proportion of IFNγ and TNFα producing Tep or Tlp cells upon peptide restimulation 8 days after priming.
Data represent 2 independent experiments with 4-5 mice per group. Error bars indicate SD. Significance was determined by t-test. *, p<0.05.
See also Figure S5.