Figure 7. Chronic viral infection redirects anti-tumor CD8⁺ T cell differentiation and control of tumor growth.

OT-I cells were injected into naïve mice or into mice infected for 21 days with Cl13. One day later EG7 tumors were injected and tumor-specific responses quantified on day 12.

(A) Total number of tumor-specific OT-I CD8⁺ T cells in tumors of naïve mice (T) or in mice infected with Cl13 (T+L).

(B) Expression of Tbet and Eomes in tumor-infiltrating OT-I T cells.

(C) Proportion and GMFI of GzmB⁺ cells and proportion of TCF1⁺ OT-I cells in tumors.

(D) Tumor size on day 12 after tumor injection in naïve and chronically infected mice that received OT-I cells or no cells.

(E) OT-I cells were injected into naïve mice (black) or mice that had been infected for 21 days with Cl13 (red). One day later EG7 tumors were injected into all mice either alone (shaded bars) or in combination with OVA-peptide pulsed DCs (open bars). Graph depicts the number of tumor-specific GzmB⁺ effector cells 14 days later.

Data represent 2 independent experiments with 4-5 mice per group. Error bars indicate SD. Significance was determined by t-test * p<0.05.

See also Figure S6.