Figure 3.

PLX reduces the number of M2 TAM in the TME of 4T1 lung lesions. (A) Regions of 4T1 lung tumor nodules (circled), obtained by H&E staining of Balb/c lung tissue, (B) representative IF images acquired by confocal microscopy for total TAM (F4/80⁺), CD206⁺, and M2 TAM (F4/80⁺ CD206⁺ overlay, with zoomed in regions-“bluish green” represents overlay), (C) effect of PLX (p.a.) +/− PTX (i.v.), 1 mg/kg each, on total TAM population, (D) impact of therapy on M2 TAM. Tumors were collected from animal lungs (n=3) and six random images were taken on each group sample, pixels for total TAM and M2 TAM were converted to area (1pixel = 0.98 μm²) and plotted in (C) and (D). PLX +/− PTX treated groups were compared with control and statistical significance was calculated by One-way ANOVA (*p <0.05, **p <0.01), data represented as mean ±SEM.