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Comparative polynucleotide-induced stimulation of the ATPase activity of the SARS coronavirus NTPase/helicase. Each 24-mer polynucleotide was added to a concentration of 1 μm. The degree of stimulation was calculated as a factor, normalized to the rate of ATPase activity in the absence of polynucleotide. Experimental conditions were: 0.5 mm ATP, 5 mm MgCl2, 50 mm Tris-HCl, pH 6.6, 50-μl reaction volume, 0.5 pmol of helicase, 10-min reaction.
