Figure 3.

2‐locus TADE suppression drives. (a) A 2‐locus TADE drive will function as a suppression drive if one of its drive alleles is “distant‐site” and located in an essential but haplosufficient female fertility gene (or any single‐sex fertility or viability gene), disrupting the gene with its presence. Alternatively, the drive allele can simply target the fertility gene with additional gRNAs, allowing a “same‐site” arrangement. (b) The time at which a 2‐locus TADE suppression drive (with one allele placed in a female fertility gene and the other allele in a same‐site configuration, with all components genetically unlinked) with a germline promoter (G) is expected to reach 99% of individuals in the population with varying introduction frequency and drive fitness. (c) As in (b), but for TADE alleles with a promoter leading to cutting activity in the germline of both sexes and in embryos of drive‐carrying females (GE). Released individuals are heterozygous for all drive alleles. Gray indicates that the drive was eliminated within 100 generations