TABLE 5.

Effect of vaccination with miR-1 inhibitor on tick feeding^a.

Parameter	Immunized with
	MsAnt control	Non-injection control	Injection Ant-1
Duration of feeding (days)	11–14	11–14	6–8**
Engorged weight, mean (mg)b	127.20 ± 2.1	119.48 ± 2.0	81.00 ± 1.9**
Spawning rate (%)	80.00	86.67	73.33
Average egg weight (mg)	52.15 ± 3.2	48.96 ± 3.1	48.51 ± 3.0
Mortality (%)c	20 ± 0.1	26 ± 0.6	23 ± 0.3

^aStatistically significant (P < 0.05) as calculated by Student’s t-test. “**” Indicated **P = 0.0044 < 0.01 (Student’s t-test). ^aValues are expressed as follows: average ± standard deviation. ^bDead ticks were excluded; calculated as batch average (batch weight/number of adult ticks). ^cMortality rate was calculated as the number of deaths during and after the feeding period.