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. Author manuscript; available in PMC: 2021 Apr 22.
Published in final edited form as: J Bone Miner Res. 2020 Feb 25;35(6):1174–1187. doi: 10.1002/jbmr.3966

Fig. 3.

Fig. 3.

IL-7 and IL-15 together lead to induction of IL-17A, TNFα or both in memory T-cells. (A) Cytokine-dependent conversion of TMEM to TEM: culturing memory T-cells (TMEM: CD3 CD44high CD62Llow) with IL-7 and IL-15 together induces TNFα and IL-17 in the absence of antigen stimulation. Representative flow contour plot of six experiments is shown. (B) Co-culturing TMEM with BMDCs induced TNFα and IL-17A in TMEM: culturing T with CFP+ BMDC in the absence of E2 results in a fourfold increase in TMEM and twofold decrease in TEFF levels relative to DC in the presence of 10nM E2; BMDC induced IL-17A and TNFα in TMEM in the absence of E2 mimicking OVX. Representative flow contour plot from six experiments is shown. (C) E2 replacement in OVX mice restores TMEM levels to that of sham-operated mice. Data from six mice/group. (D) IL-15 induces FAS in TMEM: culturing BM-TMEM with IL-15 induced FAS in this subset. (E) A model summarizing the data from Fig. 1 through Fig. 3D.