Figure 3.

Exercise-induced enhancement of miR-486a-5p and potentially downregulation of Mst1 inhibited apoptosis in cardiomyocytes. (A) Bioinformatic analysis based on 3 public databases predicted that miR-486a-5p expression is enhanced by exercise (29) and can target Mst1. (B) Relative miR-486a-5p levels assessed by RT-qPCR; *, P<0.05 vs DM. (C) miR-486a-5p levels after miR-486a-5p mimic or inhibitor transfection, as assessed by RT-qPCR; *, # P<0.05 vs Control. (D) Representative immunofluorescence images of staining with DAPI (blue) and TUNEL (green). (E) Quantitative assessments of apoptotic indexes; *, P<0.05 vs Control; #, P<0.05 vs HG; $, P<0.05 vs Control+miR mimic; §, P<0.05 vs HG+miR mimic; &, P<0.05 vs Control+miR inhibitor. (F-I) Representative immunoblots and semiquantitative analysis of biomarkers of apoptosis; *, P<0.05 vs Control; #, P<0.05 vs HG; $, P<0.05 vs Control+miR mimic; §, P<0.05 vs HG+miR mimic; &, P<0.05 vs Control+miR inhibitor. Control, negative control; miR mimic, cardiomyocytes with miR-486a-5p mimic transfection; miR inhibitor, cardiomyocytes with miR-486a-5p inhibitor transfection; HG, cardiomyocytes cultured under hyperglycaemic conditions; Control+miR mimic, control cells with miR-486a-5p mimic transfection; HG+miR mimic, HG cultured cells with miR-486a-5p mimic transfection; Control+miR inhibitor, control cells with miR-486a-5p inhibitor transfection; HG+miR inhibitor, HG cultured cells with miR-486a-5p inhibitor transfection. The data are presented as the means±SEM of triplicate experiments, and Student’s t-test or ANOVA was used to assess group differences