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. Author manuscript; available in PMC: 2021 Apr 22.
Published in final edited form as: DNA Repair (Amst). 2019 Dec 5;86:102767. doi: 10.1016/j.dnarep.2019.102767

Fig. 3.

Fig. 3.

Steady-state and pre-steady-state kinetics of TkoAGOG. A, A 60-nt, 5’-FAM (blue), 3’-ROX (red) labeled dsDNA substrate with a centralized 8oxoG was incubated with TkoAGOG at 65 °C and quenched with NaOH + SDS allowing for visualization. B, The conversion of the 60-nt substrate to the 36-nt 3’-ROX product by TkoAGOG over time. C, A representative graph of a TkoAGOG steady-state multiple turnover experiment ([DNA] > > [TkoAGOG]). The amount of product formed was calculated using the 3’-ROX labeled fragment, and data was fit to a linear line. D, A representative graph of a TkoAGOG pre-steady-state single turnover experiment ([TkoAGOG] > > [DNA]). The product formed was calculated using 3’-ROX labeled fragment, and data was fit to Eq. (1).